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Methylated DNA immunoprecipitation (MeDIP) assays

MH Masanori Hasegawa
HT Hidekazu Takahashi
HR Hasan Rajabi
MA Maroof Alam
YS Yozo Suzuki
LY Li Yin
AT Ashujit Tagde
TM Takahiro Maeda
MH Masayuki Hiraki
VS Vikas P. Sukhatme
DK Donald Kufe
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DNA methylation analysis was performed using the Methylation DNA IP (MeDIP) kit (Active Motif) by immunoprecipitating and enriching for DNA fragments containing 5-mC. Genomic DNA from CshRNA or xCTshRNA expressing cells was digested with Mse-1, heat denatured, and methylated DNA was precipitated with an anti-5-mC antibody (Active Motif). The precipitated DNA was subjected to qPCR analysis using the SYBR green master mix and amplification in an AB7000 sequence detector (Applied Biosystems). Primer sets used to analyze the MUC1 promoter are listed in Supplementary Table S2.

Copyright and License information:  ©2016 Hasegawa et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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