MCF-7-ADR cells were plated in 24-well plates and incubated with various concentrations (0.1, 0.5, 1, 5, 10 μM) of CRT0066101 for 72 h. Cell viability was determined by a WST-8 assay (Sigma-Aldrich), and optical density measured at 450 nm using a microplate reader.
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