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2.10. Assay of GLP-1 Secretion from Cells
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NCI-H716 cells (5 × 105 cells per well) were treated with imperatorin at the indicated concentration under 37 °C or 1 h. In some experiments, a 30-min incubation with triamterene was conducted prior to imperatorin treatment. The supernatants were then collected and assayed by using a GLP-1 active ELISA kit (Millipore Co., Billerica, MA, USA). Each assay was performed in duplicate for the indicated samples.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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