2.2. Plasma triglyceride assays

Angptl4^−/− mice and wild-type littermates were fasted for 4 h (fasted group) or fasted for 6 h and then fed normal chow for 2 h (refed group). Blood was collected via tail-nick into EDTA-coated collection tubes. After centrifugation at 1500×g for 15 min at 4 °C to pellet the cells, plasma from each mouse was combined with Infinity™ Triglyceride Reagent (Thermo Scientific TR22421) according to the manufacturer's instructions. Samples were incubated at 37 °C for 10 min and absorbance was measured at 500 nm. Triglyceride concentrations were determined by comparison to a standard curve prepared from a triolein standard (Nu-Chek Prep, Lot T-235-N13-Y).