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Total cell lysates were analyzed by western blotting as previously described (17). The antibodies used were listed in Supplementary Table S4. An enzyme-linked immunosorbent assay (ELISA) was used to detect TRAIL secretion ability using a commercial TRAIL ELISA kit (R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
Copyright and License information: ©2017 The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
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