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NF-κB activation assay

SS Suman Suman
TD Trinath P. Das
SS Suman Sirimulla
HA Houda Alatassi
MA Murali K. Ankem
CD Chendil Damodaran
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To measure the level of NF-κB activation in pCMV/HCT-116 and AKT/HCT-116 cells, nuclear extracts were prepared using N-PER-Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, Rockford, IL, USA) according to the manufacturer's protocol. A TransAM NF-κB family transcription factor assay kit (Active Motif, Carlsbad, CA, USA) was used to detect NF-κB activation according to the manufacturer's protocol.

Copyright and License information:  ©2016 Suman et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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