Experiment 3: Investigation of mPFC inputs from vHP GLP-1R expressing neurons

Rats (n=4) received a unilateral 100nl pressure injection of fluorogold (FG, Fluorochrome LLC; 2% in 0.9 % NaCl) targeting the mPFC. Following a survival period of 10 days, animals were fixation-perfused and tissue was harvested and processed as described in the Methods section. A representative animal with fluorogold back-labeled neurons in the vHP (field CA1) was selected for GLP-1R FISH analyses (See Methods and Supplementary materials). In order to observe separate GLP-1R mRNA in vHP cell bodies (as fluorogold and DAPI autofluoresce in the same channel), the protocol above was repeated in a separate untreated animal where DAPI (ACD, 320851) was placed on the slide prior to coverslipping.