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Procedure for the Installation of Branching β1,6 GlcNAc (5, 23).

AP Anthony R. Prudden
LL Lin Liu
CC Chantelle J. Capicciotti
MW Margreet A. Wolfert
SW Shuo Wang
ZG Zhongwei Gao
LM Lu Meng
KM Kelley W. Moremen
GB Geert-Jan Boons
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HMO (4 or 9) and UDP-GlcNAc (1.5 eq per GlcNAc added) were dissolved at a final HMO concentration of 10 mM in a Mes buffer (50 mM, pH 7.0) containing Na2EDTA (10 mM). To this mixture, CIAP (10 U μL−1) and GCNT2 (7.5 μg per μmol HMO) were added. Following purification, the respective products (5, 78%, and 23, 74%) were obtained as white solids.

Copyright and License information:  ©2017-06-19

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