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HIV-1 latency reversal in ACH2 and U1 cells.
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The ACH2 (22) and U1 (23, 24) latently HIV-1-infected CD4+ T cell lines were seeded in cRPMI in 96-well plates (Nunc, Denmark). Cells (2 × 105 cells/well) were coincubated with MGN1703, CpG-ODN2006, or culture supernatants from MGN1703-stimulated PBMCs for 48 h in triplicates. Virus was inactivated with 1% Empigen (Albright & Wilson UK Ltd.), and an HIV-1 p24 enzyme-linked immunosorbent assay (ELISA) was performed on supernatants and lysates as described previously (25).
Copyright and License information: ©2016, American Society for Microbiology. All Rights Reserved.
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