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Quantitation of PI3Kα lipid kinase activity was carried out by Life Technologies (Madison, WI) with purified enzyme using the fluorescence-based Adapta™ TR-FRET assay (assay-# 1014) protocol. Assays were conducted in triplicate with various inhibitor concentrations (0.1 nM – 10 μM).
Copyright and License information: ©2017
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