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Measurement of plasma BUN, creatinine, and glucose
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Mouse blood was collected from the tail vein of mice on the day of sacrifice. Blood samples were centrifuged at 8000 rpm for 8 min and plasma transferred to 1.5 mL plastic Eppendorf tubes, stored at −20°C until analysis. Plasma creatinine and blood urea nitrogen (BUN) were measured using the Creatinine FS kit and Urea FS kit from DiaSys Diagnostic Systems, Germany. Plasma glucose was measured using Glucose GOD FS kit (DiaSys) according to the protocol provided by the manufacturer.
Copyright and License information: ©2017 The Authors. published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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