NF-kB, p65 assay kit for the determination of NF-kB activity

Nuclear protein extracts were used to determine NF-kβ activation with an NF-kB activation assay kit (FIVEphoton Biochemicals) according to the manufacturer’s protocols. Cells were fractionated into cytoplasmic and nuclear fractions, which were then subjected to Western blot analysis using an antibody selective for NFκB, p65.

Statistical analysis was carried out using Microsoft Excel Data Analysis tool. Data are expressed as mean ± SE of three independent experiments. Statistical significance was determined by t test. *P<0.05.