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We performed OCT using commercial equipment (Spectralis, software version: 1.8.6.0; Heidelberg Engineering, Inc., Carlsbad, CA, USA) anterior segment mode. Organoids were imaged while suspended in 0.1 mL of culture media in 0.2 mL polypropylene tubes suspended in the device's imaging plane. High-definition horizontal raster scans were acquired of each sample, using parameters in Supplementary Figure S6.
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