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Vasculogenic mimicry formation assay
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Vasculogenic mimicry formation assay was performed using a commercialized Matrigel assay kit (BD Biosciences). ECM Matrigel (200 μL) was added to 48-well tissue culture plates and then incubated at 37°C for 2 hours. Cells were transfected with pre-miR-200c or control 1 day before seeding onto coated plates. After growth for 24 hours on the plate, vasculogenic mimicry formation was assessed using an inverted microscope.
Copyright and License information: ©2017 Koo et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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