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shRNA-mediated knockdown cells were generated as described (27). To generate knockdown cells, retroviral shRNA constructs (shNS and shG9a) with viral packaging plasmids (pMD2.G and pMD-MLV) were transfected into HEK293T cells. Viral supernatant was collected 3 d after for target cell infection.
Copyright and License information: ©2017
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