The LLC or B16 tumour progression was monitored using Imaging System Xenogen IVIS 200 (Advanced Molecular Vision, Caliper Lifesciences, Waltham, MA, United States). 100 μl of luciferin potassium salt (30mg/ml) was intraperitoneally injected in tumour bearing DUSP3+/+ and DUSP3-/- mice. After 12 minutes, the photons emitted by the luciferase activity were detected. The bioluminescence was quantified using the Living Image Software (Caliper Life Sciences, Waltham, MA, United States) and by delimitation of region of interest (ROI) around the lungs.
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