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Assays of Mcl-1 expression and glutathionylation
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Mcl-1, Bcl-xl and Bcl-2 protein levels were determined by western blot as previously described [44]. Cellular glutathionylation of Mcl-1 was determined by IP with an anti-GSH antibody under non-reducing conditions, followed by western blot analysis [7, 18] using an anti-Mcl-1 antibody. Antibodies to Bcl-2 and Bcl-xl were purchased from Santa Cruz. Antibodies to GSH and Mcl-1 were purchased from Abcam.
Copyright and License information: ©2016 Li et al.
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