3.12. Glucose Uptake in HepG2-Cells

Cells were exposed to test samples dissolved in DMSO and diluted in the HepG2 cell medium and incubated for 24 h. The final DMSO concentration was 0.1% in both the DMSO control and test samples. Thereafter, cells were exposed to d-[¹⁴C(U)]glucose (1 μCi/mL, 100 μM) for 4 h. A 96-well UniFilter-96 GF7B microplate was mounted on top of the CellBIND plate and CO₂ production was measured. After incubation the cells were washed twice with ice-cold phosphate buffered saline (PBS), lysed in 0.1 M NaOH, and radioactivity measured by liquid scintillation counting [63]. The protein content of each sample was determined and the glucose uptake was calculated using protein levels for standardization [45].