The human hepatoblastoma cell line HepG2 (HB-8065; ATCC, Manassas, VA, USA) was cultured in DMEM-Glutamax™ (5.5 mM glucose) supplemented with 10% fetal bovine serum, streptomycin (100 μg/mL) and penicillin (100 units/mL) at 37 °C in 5% CO2.
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