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Ethylene measurements on Arabidopsis seedlings were performed as previously described (Mira et al., 2016b). Briefly, 200 mg fresh weight of seedlings was incubated in a sealed 3-ml syringe for 3 h in the dark at 22 °C. The gas (1 ml) accumulated in the headspace was analysed with a Bruker 450-GC Gas Chromatograph. Data analysis was carried out using the Bruker Compass Data analysis 3.0 software. All experiments were performed in triplicate.
Copyright and License information: ©2017 The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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