Genome annotation of maize (B73, 6a) contains 40,602 predicted protein-coding genes [63]. We excluded genes with atypical lengths of 5′ UTR (below 20 nt or above 1000 nt long), genes without full-length mRNA support, without valid start and stop codon, or no PFAM annotation. This filtering resulted in 16,180 putative corn TSS.
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