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Methylation-specific PCR (MSP) analysis
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MSP was performed as previously [43]. Briefly, Genomic DNA was isolated from Hep-2 cells and pre-cleaned. The primers were used for unmethylated MGMT promoter as follows: forward: 5′-GGTCGTTT GTACGTTCGC-3′; reverse: 5′-GACCGATACAAAC CGAACG-3′. For methylation primers: forward: 5′-GT AGGTTGTTTGTATGTTTGT-3′; reverse: 5′-AACCAATA CAAACCAAACA-3′.
Copyright and License information: ©2016 Wu et al.
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