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2.3. Histopathological Procedure

BC Bo Yoon Chang
YJ Young Suk Jung
CY Chi-Su Yoon
JO Jun Seok Oh
JH Jae Heoi Hong
YK Youn-Chul Kim
SK Sung Yeon Kim
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Liver tissues were placed in plastic cassettes and were immersed in 4% paraformaldehyde for 6 h. The fixed tissues were processed as described previously [19]. The degree of hepatocellular damage was evaluated by measuring the area of necrosis in liver sections stained with hematoxylin and eosin. For this purpose, we used light microscopy (Olympus BX51, Tokyo, Japan). The necrotic zones were manually selected and the percentage of the necrotic area was determined using Cell v3.1 software, Olympus Soft Imaging Solutions (Münster, Germany).

Copyright and License information:  ©2017 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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