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Cyclic GMP (cGMP) levels were quantitatively measured by using an ELISA (enzyme-linked immunosorbent assay) kit (Abcam, 330 Cambridge Science Park, Cambridge, CB40FL, UK) according to the manufacturer's instructions as described previously [44]. and the concentration was expressed as picomoles per mg of protein. All the ELISA experiments were repeated at least four times.
Copyright and License information: ©2017 Sarkar et al
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