Carrageenan-induced air pouch inflammation model in mice

Male ICR mice (20-25 g) were purchased from Orient Bio Inc. (Seongnam, Korea). Animal study was scheduled as shown in Figure 1. To make space (or air pouch) under skin, 2-mL air was inserted three times into the intrascapular area of mice's back for 6 days. Animals were divided into two groups. Animals (n = 6) in the first group were not treated with carrageenan. Animals (n = 24) in the other group were treated with carrageenan. The animal study was repeated once with the same protocol. Carrageenan-treated group consisted of four subgroups: (1) inflammation-induced group was injected with carrageenan after normal saline oral administration, (2) 5 mg/kg indomethacin-treated group (indomethacin was used as an anti-inflammatory drug), (3) 30 mg/kg ECCF-treated group, and (4) 300 mg/kg ECCF-treated group. Indomethacin and ECCF were orally administered for 6 days. At 2 h after treatment, carrageenan solution (1 mL, 2 w/v% dissolved in saline) was injected into the air pouch.

Schematic diagram illustrating the schedule for the preparation of carrageenan-induced air pouch inflammation mouse model and oral pretreatment with indomethacin (5 mg/kg) or ECCF (30 or 300 mg/kg)