Phospho-receptor tyrosine kinase array

TL Tzu-Chi Lin
SC Syue-Ting Chen
MH Min-Chuan Huang
JH John Huang
CH Chia-Lang Hsu
HJ Hsueh-Fen Juan
HL Ho-Hsiung Lin
CC Chi-Hau Chen
request Request a Protocol
ask Ask a question
Favorite

A human phospho-receptor tyrosine kinase (p-RTK) array kit including 49 RTKs was purchased from R&D systems. si-Control and si-GALNT6-transfected ES-2 cancer cells were cultured until confluence on 10 cm2 culture plates. Cells were lysed and 250 μg of protein were used for Western blotting according to the manufacturer's protocol. Activated receptors were matched according to the phospho-RTK array coordinates.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A