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Resorption Pit Assay for Functional Osteoclast Analysis
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On day 14, cells were removed with 10% bleach for 10 minutes at room temperature. The wells were washed twice with distilled water and dried for 3 to 5 hours at room temperature. Afterward, resorption pit images were taken using a light microscope. Images were converted to black and white binary images for quantification of pit area using ImageJ.
Copyright and License information: ©2017 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
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