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The yeast (Saccharomyces cerevisiae) two-hybrid constructs were made in the pDEST-GBKT7 and pDEST-GADT7 Gateway vectors (Rossignol et al., 2007). The DNA fragment of the N-terminal segment I of ACA10 was cloned into the pCR8 TOPO TA vector and transferred to the pDEST-GBKT7 vector to obtain the BD:ACA10-I construct. The AD:BON1, AD:BON1-A, and BD-BAP1 constructs were previously described (Hua et al., 2001). The yeast two-hybrid assay was performed as previously described (Hua et al., 2001; Li et al., 2010).
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