Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Confirmation of RVLM microinjections

HR Hiviny de A. Raquel
GM Gustavo S. Masson
BB Barbara Falquetto Barna
NZ Nágela G. Zanluqui
PP Phileno Pinge-Filho
LM Lisete C. Michelini
MM Marli C. Martins-Pinge
request Request a Protocol
ask Ask a question
Favorite

At the end of the experimental protocols, the rats were euthanized with an overdose of sodium pentobarbital. Sites of RVLM administrations were marked by microinjection of Evans blue dye (2%/100 nL). Brain was removed and stored in 10% formaldehyde for subsequent histological analysis. Sequential slices (40 μm) of brainstem were cut in a cryostat, placed in gelatinized slides and stained with 1% neutral red. The sections were examined microscopically with the aid of a rat brain atlas (Paxinos, 1998). Only rats with confirmed RVLM microinjection were included in experimental groups (see Figure 2F).

Copyright and License information:  ©2016 Raquel, Masson, Barna, Zanluqui, Pinge-Filho, Michelini and Martins-Pinge.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A