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Probe specific to BCSP31 gene of Brucella was designed in Genetool using gene information available in NCBI (accession no. KF56031). Sequence homologies with other organisms were ruled out by using NCBI blastn program. The possibility of dimer and hairpin loop formation of oligonucleotide were minimised by analysing the sequence in Integrated DNA technology (IDT) online Oligoanalyser software.
Copyright and License information: ©2017 Pal et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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