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To assess Mtb survival in vitro, BMDMs from C57BL/6 mice, unless otherwise specified, were seeded 1 d before infection and infected with a single-cell suspension of Mtb at a MOI of ∼3, as previously described (24) and detailed in SI Materials and Methods. RAW264.7 cells were used when macrophages were transfected with CpsA as described in SI Materials and Methods.
Copyright and License information: ©2017
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