2.2. EoL-1 cell culture

Cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, l-glutamine, and antibiotics (50 U/mL penicillin and 50 μg/mL streptomycin) in a humidified atmosphere at 37 °C with 5% CO₂. Undifferentiated EoL-1 cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum in 5% CO₂ at 37 °C. Cells were induced to differentiate via the addition of n-butyrate (0.5 mM), IL-3 (1 ng/mL), IL-5 (1 ng/mL) and GM-CSF (0.5 ng/mL) for 5 days [9]. The culture medium was changed at day 1 and every 2 days thereafter. The cell concentration was adjusted to 1 × 10⁶ cells/mL for chemotaxis assay.