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Leishmania (L.) amazonensis promastigotes
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Promastigotes of Leishmania (L.) amazonensis LV79 (MPRO/BR/72/M 1841) and PH8 (IFLA/BR/67/PH8) strains were cultured at 24°C in M199 medium supplemented with 10% fetal calf serum (FCS). Parasites were sub-cultured every 7 days to inoculums of 2 × 106/mL.
For differentiation of amastigotes into promastigotes, lesion-derived parasites were counted using Neubauer chamber and transferred to M199 medium with 10% FCS at densities of 103, 104 and 105 parasites/mL. Cells were incubated at 24°C for 4 days and promastigote densities were determined.
Copyright and License information: ©2017 de Rezende et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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