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SLN characterization techniques

AM Ana Raquel Madureira
SN Sara Nunes
DC Débora A Campos
JF João C Fernandes
CM Cláudia Marques
MZ Monica Zuzarte
BG Beatriz Gullón
LR Luís M Rodríguez-Alcalá
CC Conceição Calhau
BS Bruno Sarmento
AG Ana Maria Gomes
MP Maria Manuela Pintado
FR Flávio Reis
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Particle size, polydispersity index, and zeta potential were measured using dynamic light scattering (DLS) ZetaSizer NanoZSP (Malvern Instruments, Malvern, UK). Zeta potential was measured using laser Doppler anemometry. All analyses were carried out at an angle of 90°C at 25°C.

Centrifugation at 4,200× g (20 minutes at 4°C) using centrifugal filter units with a cutoff of 10 K (Amicon® Ultra-4, Millipore, MA, USA) was performed to separate nonen-trapped compounds from SLN aqueous solution. The RA concentration was quantified in the resulting supernatant by HPLC analysis as previously described.6 The association efficiencies of RA and extracts (AE%) were calculated according to the following formula:

where AE% is the association efficiency percentage and PC is phenolic compound (RA in the case of SLN-RA).

Copyright and License information:  ©2016 Madureira et al. This work is published and licensed by Dove Medical Press Limited
The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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