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Nitrocefin assay to determine β-lactamase activity
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50 μl of samples were added in triplicate to a 96-well plate. Nitrocefin was diluted to 0.5 mg/ml in PBS and 50 μl was added to each sample. The absorbance at 486 nm was measured in the FluoStar Omega plate reader for 2 h, and the change in absorbance over time was used to determine the specific activity in samples, using the protein concentration determined by the CBQCA kit.
Copyright and License information: ©2017 O’Donoghue et al
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