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2.4. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
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SDS-PAGE (12%) was run as described by Laemmli [18], with modifications described by Helmerhorst and Wei [19]. Protein molecular weights were estimated using standard protein markers (97–14.4 kDa; GE Healthcare, Amersham LMW, UK). Gels were stained with Coomassie Brilliant Blue R-250.
Copyright and License information: ©2017 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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