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RF Rie Furuta
JY Jun-ichirou Yasunaga
MM Michi Miura
KS Kenji Sugata
AS Akatsuki Saito
HA Hirofumi Akari
TU Takaharu Ueno
NT Norihiro Takenouchi
JF Jun-ichi Fujisawa
KK Ki-Ryang Koh
YH Yusuke Higuchi
MM Mohamed Mahgoub
MS Masakazu Shimizu
FM Fumihiko Matsuda
AM Anat Melamed
CB Charles R. Bangham
MM Masao Matsuoka
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HTLV-1 transformed human T-cell line (MT4, HPB-ATL-2) and HTLV-1 negative human T-cell lines (Jurkat, CCRF-CEM) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS)(Biowest) and antibiotics. MT-4 and HPB-ATL-2 cells were gifts from Dr. Isao Miyoshi (Kochi University) and Dr. Shigeru Morikawa (Shimane University) respectively. Jurkat cells were obtained from Dr. Shimon Sakaguchi (Osaka University). JET WT35 is a subline of Jurkat cell expressing tdTomato under the control of 5 times tandem repeat of Tax responsive element (TRE)[45]. They were cultured with RPMI 1640 medium supplemented with 10%FBS, antibiotics and G418 (250 μg/mL) for selection.

Copyright and License information:  ©2017 Furuta et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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