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Phylogenetic analysis

JL Jian-Nan Liu
ZY Zhi-Jun Yu
LL Li-Meng Liu
NL Ning-Xin Li
RW Rong-Rong Wang
CZ Chun-Mian Zhang
JL Jing-Ze Liu
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Sequence of FLEs-Hd was compared with known sequences listed in the GenBank nucleotide sequence databases. The gram-positive bacterium Bacillus subtilis (X60646) was used as an outgroup species. The sequences obtained were aligned using the CLUSTAL W program and manually checked with excluded DNA gaps52. The phylogenetic trees were produced according to the neighbor-joining method after Kimura 2-parameter correction in the MEGA version 6 using bootstrap analyses with 1000 replicates53.

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