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RNA extraction, cDNA synthesis and qPCR
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Total RNA was isolated using RNeasy mini kit (Qiagen). cDNA was synthesised using SuperScript III (Invitrogen) and oligo-dT primers. qRT-PCR was performed with TaqMan Gene Expression assays (Thermo Scientific) using probes listed in Table S7.
Copyright and License information: ©2017 2017. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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