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RNA extraction and reverse transcription-polymerase chain reaction (RT-PCR)
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Total RNA from human adipose arteries and arterioles (200–500 μm) was extracted and cDNA was synthesized. The cDNA was amplified using a touch-down PCR protocol with gene-specific primers. Relative KVα1.5 gene expression was quantified with real-time PCR using pooled sample cDNA from each non-CAD and CAD group. For primer sequences refer to Online Table III.
Copyright and License information: ©2016
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