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Non-homologous end joining (NHEJ) assay
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The effect of SLs on NHEJ was analyzed via a U2OS EJ5-GFP reporter system [44]. Cells were transfected with pCBASce, an empty vector, pCAGGS, as a negative control or full-length GFP expression construct (NZF-GFP) as a positive control for GFP+ cells. On the next day, cells were treated with the indicated concentrations of ST362 and MEB55 and after an additional 24 hr, cells were processed for flow cytometric analysis. For each analysis, 1×104 cells were collected. Each data point represents the mean ± standard deviation from three independent experiments.
Copyright and License information: ©2016 Croglio et al.
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