Rotarod test

Male mice were tested for their ability to balance on the rotarod (3 cm diameter; rate of rotation, 16 revolutions per minute) after i.c.v., i.t. or i.v. administration of BN‐9 or vehicle. Prior to drug administration, animals were trained in three consecutive sessions to stay on the rod and reach the cut‐off time of 300 s. Animals that did not remain on the rod for the 180 s were not used for the experiments. After 2 days of training, three times a day, mice were tested at 10, 20, 30 and 40 min after administration of the vehicle or drug. The latency to fall off the rod was recorded at each time point. Results are expressed as the time that mice stayed on the rotarod for each drug treatment.