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Cells were transiently transfected with 50 nM of a pool of 4 different siRNAs against Akt1 and/or 25 nM of a pool of 4 different siRNAs against Rad51 or an equal concentration of the non-targeting siRNA using Lipofectamine 2000. Whole cell lysates were prepared 48 h and 72 h after transfection to analyze the knockdown efficiency by Western blotting.
Copyright and License information: ©2017 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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