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The microsatellite instability (MSI) status of each of the cell lines was determined using the MSI analysis system (Version 1.2, Promega) according to the manufacturer's instructions. Fragment analysis was performed on an Applied Biosystems 3130 Genetic Analyser (Life Technologies). Samples with at least two out of five unstable mononucleotide markers were classified as MSI-H. TGFBR2 and ACVR2 microsatellite analysis was performed as described previously [21].
Copyright and License information: ©2016 Boot et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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