siRNA transfection

ZZ Zili Zhai
WL Weimin Liu
MK Manjinder Kaur
YL Yuchun Luo
JD Joanne Domenico
JS Jenny Mae Samson
YS Yiqun G Shellman
DN David A Norris
CD Charles A Dinarello
RS Richard A Spritz
MF Mayumi Fujita
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Cells were transfected with 40 nM NLRP1 siRNA (a mixture of two preselected siRNAs for NLRP1) or negative control (Qiagen Valencia, CA) using Lipofectamine 2000 reagent in OPTI-MEM1 reduced serum medium overnight.

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