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Rolling circle amplification (RCA)
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RCA was performed according to the manufacturer instructions of Templiphi 100 amplification kit (GE Healthcare Life Sciences, Pittsburgh, USA) using 100 ng of purified genomic DNA from the virus-challenged cowpea plants. The RCA amplified products were digested with MfeI, unique restriction site present in the genome of MYMIV DNA-A and DraI, present in the genome of DNA-B and the digested products were resolved on 1% agarose gel.
Copyright and License information: ©2017 Kumar et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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