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Samples were fixed/perfused with a 0.05% glutaraldehyde solution, embedded in Epon-812, cut in thin sections (600–900 nm), and stained with Reynolds lead citrate. Electron microscopy images were acquired under a Philips Tecnai-12 electron microscope (Philips, Eindhoven, The Netherlands).
Copyright and License information: ©2016 the American Physiological Society
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