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2.11. Determination of Collagen Content

BL Bo Li
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A commercial hydroxyproline assay kit (Nanjing Jiancheng Bio Inst., Nanjing, China) was used to analyze the Hyp content. Briefly, about 0.05 g skin tissue was totally hydrolyzed, oxidized and reacted with dimethyl-amino-benzaldehyde. The end product has a maximal absorption at 550 nm. The Hyp content in the skin was finally determined by comparison with the absorbance of the Hyp standard. The collagen content was calculated according to the Hyp content using a conversion factor of 8.00 [25].

Copyright and License information:  ©2017 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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