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Apoptosis detection was performed through the analysis of Annexin V/PI staining (Roche, 11 988 549 001). Fluorescence detection was performed with a FC500 flow cytometer (Beckman Coulter, Milan, Italy). Apoptosis was also assessed evaluating apoptotic markers by immunoblotting analysis. In particular the cleaved forms of caspase 3 and PARP were detected together with the up-regulation of NOXA (ABCAM, ab13654).
Copyright and License information: ©2017 Taylor & Francis Group, LLC
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