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Long-term viability assay was performed by colony formation assay. In brief, cells were seeded in 6-well plates and allowed to adhere overnight then treated with DMSO control (0.1% v/v) or LY2835219. Media were changed every 3 days with fresh reagents added. Following 10-day treatment, cells were fixed and stained with crystal violet. Colonies with > 50 cells were quantified using ImageJ (NIH, Bethesda, MA).
Copyright and License information: ©2016 Ku et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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